Mixing dual and single index TruSeq samples in a single MiSeq run

pmiguel

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Join Date: Aug 2008

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#1

Mixing dual and single index TruSeq samples in a single MiSeq run

12-20-2012, 04:37 AM

Should be possible, right? What happens if you specify both in a sample sheet?

I presume that if you specify a mixture of 6 and 8 base indexes for index1 that the instrument will do 8 bases. Then a mixture of 8 and zero base indexes for index1 should also give the desired result.
Tags: dual indexing, indexing, miseq, truseq
Vinz

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Join Date: Dec 2010

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#2

12-21-2012, 06:21 AM

I would extend the 6 bases of index 1 to 8 bases. The 2 bases after the index are known...
Likewise I would also give the 8 bases of the adapter for the single index samples. That way you do not rely on the software to be very advanced...
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Mixing dual and single index TruSeq samples in a single MiSeq run

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