I will be crazy: Help about SNPs and tree!!!!

[mm.perrineau]

Hi,

Somebody can help me????
I will be crazy: I am not bioinformaticien and i have to make a phylogenetic tree reflecting the genetic distance between 4 strains...
I have one strain well sequenced and his genome is annotated...
I have all reads mapped on this genome for the 3 others strains...

I used CLC for the SNPs calling and now i am stopped since few weeks and i become crazy!!!!!!!!
I need to build a simple tree reflecting the genetic distance between those 4 strains...
Can you help me...

And i need too some software to make some statistical tests on the SNPs... Do i have some genomic regions ? some genes under selection pressure ?

Thank you very much !!!!!

And please help me i am totally desperate !!!!

[khoa128]

Hi
you to be used software of this: NextGENe® software for Next Generation (NGS) Sequence Analysis. You may download a 30 day trial from our ftp site:

ftp://ngval:[email protected]_35Days.EXE

[AdrianP]

Hello,

I do not mean to advertise my own thread, but it seems like we are doing something similar. Check out what I have done so far and my obstacles:

http://seqanswers.com/forums/showthread.php?p=112840