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What is the difference and how does it work for illumina-solexa?
Thanks
Darshan
Thanks
Darshan
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A library for an Illumina sequencer consists of a set of fragments of around 200-700bp with specific adapters ligated onto the ends. A basic single end run would use a primer to one of those adapters to sequence a short fragment (~30-100bp) from one end of the fragment. In a paired end run you can use a second primer to the other adapter to get a corresponding sequence from the other end of each fragment, thus you would end up with two sequences for every fragment, one from each end.
If you have paired end data you can therefore infer the full length of each fragment if you are mapping it back against a known reference sequence. You also have more information which can be used when mapping the sequence to the reference.
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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