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Combine 454 data with Illumina?
Aloha,
First post. I am new to bioinformatics.
I'm calling and comparing SNPs between a few different populations sequenced with Illumina. I'd like to incorporate an additional library of existing data but it was sequenced with 454. Advice?
My concerns with the 454 data are:
*Accounting for homopolymer errors
*Longer reads (and reads of varying lengths)
*Lower coverage than the other libraries
...nevertheless, it'd be nice to use the data. What's the best way to do this?
First post. I am new to bioinformatics.
I'm calling and comparing SNPs between a few different populations sequenced with Illumina. I'd like to incorporate an additional library of existing data but it was sequenced with 454. Advice?
My concerns with the 454 data are:
*Accounting for homopolymer errors
*Longer reads (and reads of varying lengths)
*Lower coverage than the other libraries
...nevertheless, it'd be nice to use the data. What's the best way to do this?