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Hi guys,
I'm relatively new to this field and I'd like a few advices on how I should proceed to get the best assembly I can of a 6 Gb genome. We have about $100K to spend within 2 years, and absolutely no genomic data for this species.
The best plan I've got so far is to start spending money this year doing a first survey of the genome at 30x, using a few mate pair libraries and maybe a similar approach to the one developed by 'Young_etal_GenomeRes2010_A new strategy for genome assembly using short sequence reads and reduced representation libraries'.
Next year I would spend the rest on the money, hoping that Moleculo or other approaches for long fragment sequencing will be available by that time.
Thanks for your help.
I'm relatively new to this field and I'd like a few advices on how I should proceed to get the best assembly I can of a 6 Gb genome. We have about $100K to spend within 2 years, and absolutely no genomic data for this species.
The best plan I've got so far is to start spending money this year doing a first survey of the genome at 30x, using a few mate pair libraries and maybe a similar approach to the one developed by 'Young_etal_GenomeRes2010_A new strategy for genome assembly using short sequence reads and reduced representation libraries'.
Next year I would spend the rest on the money, hoping that Moleculo or other approaches for long fragment sequencing will be available by that time.
Thanks for your help.