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Cancer panel v2,low quality,316v2
Hi, these days ,I run some 316v2 chips on PGM, and the %low quality show very high, ranged from 27-40%! Can anyone tell me why?
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We run a Proton and found that we improved our quality by reducing the quantity of DNA that goes into the emPCR by by 20%, we now do this is standard
JPC -
Thank you for your reply!Originally posted by JPC View Post
We had try that before, but it didn't work!
by the way, how do you quantify the library, 2100 or qubit?Comment
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qubit for quant, but we also use the bioanalyser tp make sure the profile looks as expected (no primer etc.)
JPCComment
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You probably need to check amplifiability of your DNA. Using the RNaseP kit will help out with that. Crap in, crap out.Comment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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