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Senior Member
Join Date: Nov 2009
Location: SEA
Posts: 114
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This question might be better directed to SOLID developers
But I have a problem where my final basespace output I have just a fasta header and no sequence. Did the solid scripts remove it because of low quality? These are the last few steps. velvet_0.7.55/velvetg_de ./S4output_directory/ -read_trkg yes -amos_file yes solid_denovo_postprocessor.pl --afgfile ./S4output_directory/velvet_asm.afg --csfasta ./S4-output/colorspace_input.csfasta --run fragment --output ./sample4_input echo 'Running denovoadp' time denovoadp ./sample4_input 30000 > ./sample4_output so after step 1 I get this in contigs.fa >NODE_780_length_22_cov_13.590909 CCCCCCCCCCCCCCCCCACATGGCCGCGCCCCCCCCCCCCCC from step 2 onwards the contig has empty sequence i.e. >NODE_780-0 >NODE_1882-0 |
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