New IonTorrent Website

[IonTorrent]

Come visit the new IonTorrent Website at

http://www.iontorrent.com/

And read about the Ion Torrent Personal Genome Machine Sequencer Grant Program.

Jonathan Rothberg talks at AGBT this Saturday afternoon.

[ScottC]

Looking forward to seeing what's in the box... :-)

http://twitpic.com/15b55y

(this is not my picture, by the way)

[kainsteven]

Great presentation at AGBT, looking forward to the Ion Torrent story as it emerges over the next few months.

Steve

[greigite]

site is flashy but how about some details, like read length, run time, error rate, read length, sample prep, etc, etc...

[ScottC]

Yeah, I think it's a bit light-on with the details, but I don't think they even have a projected (or at least publicly acknowledged) release date, do they?

But there are a few bits-and-pieces around, though I haven't seen any official specs.

For example, from:

http://www.genomeweb.com/sequencing/ion-torrent-systems-presents-50000-electronic-sequencer-agbt

Comes this:

"In a proof-of-concept experiment in collaboration with the Broad Institute, Ion Torrent sequenced the 34-kilobase adenovirus genome at 59-fold coverage, dubbed by the Broad's Chad Nusbaum the "first post-light genome." The technology covered 100 percent of the genome with a consensus accuracy of 99.9999 percent, equivalent to a quality score of 61.The company has also sequenced the 4.6-megabase E. coli genome at 12.9-fold coverage, covering more than 99 percent of the genome with more than 99.9 percent accuracy. Rothberg said that the sequence showed no systematic error and that coverage across the genome was even. In addition, Ion Torrent has sequenced 841 megabases from a large genome — 3,123 exons from 207 genes — and has other collaborative work in progress. Rothberg listed both the Broad Institute and the Stanford Genome Technology Center as collaborators."

Scott.

[greigite]

they do have a contest to give away a couple machines shown on the site. it's a little difficult to come up with proposals without a few more details.

[drio]

Come visit the new IonTorrent Website at

http://www.iontorrent.com/

And read about the Ion Torrent Personal Genome Machine Sequencer Grant Program.

Jonathan Rothberg talks at AGBT this Saturday afternoon.

I am not sure if you get this very often but, the similarity between part of your logo and the Playstation logo screams for a lawsuit.

[flxlex]

Maybe its me and my lack of googling skills, but I cannot find out if the technology is a true single-molecule sequencing system, or if there is amplification involved. Does anybody know?

[seqAll]

Maybe its me and my lack of googling skills, but I cannot find out if the technology is a true single-molecule sequencing system, or if there is amplification involved. Does anybody know?

The article cited by ScottC:
"At the heart of the desktop-size sequencer is a 9 by 9 millimeter semiconductor chip, currently consisting of an array of about 1.55 million 3.5-micrometer wells ...

... a future generation of the chip, shrinking the wells to 1.3 micrometers, increasing the number of wells and sensors to several hundred million, and speeding up DNA synthesis to less than two seconds per base, from the current rate of four seconds per base...

...To sequence DNA, amplified template is loaded into the wells"

So, it is not a single-molecule sequencing system. But I am really facinated by this system than other 3rd-gen, can't wait to buy one.

[GW_OK]

In talking with the reps at AGBT it is definitely clonal amplification on a solid substrate. They said they're trying out some different things but unless they've got something way up their sleeve I'll bet it's emulsion PCR based sample prep.

[IonTorrent]

Thanks for everyone's interest. The instrument is not launched yet. We are currently in a "technology preview" mode. More details will be forthcoming.