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I have a few total RNA samples that I would like to prepare for the Illumina GA platform, however they are just below the threshold stated in the protocol for processing for mRNA-seq. They recommend 1-10ug total RNA, but I have 800ng in my sample. Has anyone validated this protocol for lower amount of starting material (total RNA)? I would like to avoid pre-amplification of my RNA if possible. Cheers!
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM