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Old 07-13-2014, 08:37 AM   #1
Location: Portugal

Join Date: Apr 2011
Posts: 50
Default Bubbles in Miseq Flowcell

Hi everyone,

I would like to know your opinion about the images in attachment.
The images are from different Runs, but this is the 5th Run that we detected strange things like this bubble.
I don’t know if you check the images in every Run, but for you, what kind of strange things you find in the images and assume that its normal?


Last edited by DRYTCYV; 07-13-2014 at 08:47 AM.
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Old 07-13-2014, 11:03 PM   #2
Location: Germany

Join Date: Jan 2013
Posts: 46

I observed similar things as well. These runs more or less failed with bad clustering efficiency. Illumina support is on the case but hasn't solved anything, yet (and I don't know whether the bubbles are the cause).
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Old 07-15-2014, 07:15 AM   #3
Location: Portugal

Join Date: Apr 2011
Posts: 50

Hi dfhdfh,

Thank you for your feedback.
We alreday runned the Miseq test and there's no problem in the fluidic system, but our local support will be here tomorrow to change some part from the fluidic system.

No more feedback? anyone?
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Old 07-18-2014, 05:07 PM   #4
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Location: USA

Join Date: Oct 2008
Posts: 158

Bubbles are not that uncommon. It really depends on the frequency. I'd be curious if you find any tips.
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