Hello party people,
Does anyone know why Illumina's 1st strand synthesis protocol (for RNA-seq) uses Superscript II (SSII, Invitrogen) as opposed to Superscript III (SSIII)?
Besides it lower thermal stability and shorter half life, SSII exhibits tailing activity, adding "2 to 3 bases in a template independent fashion to the end of a primer." (taken from invitrogen product notes)
I am a novice, still learning about the seq technology and illumina protocols so bear with me. Shouldn't introducing these extra bases effectively decrease the length of your final sequence read?
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