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Old 04-01-2010, 08:35 AM   #1
mjouret
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Default Whole genome assembly of cDNA with Illumina paired-end sequencing

Does anybody know an opensource software package for whole genome assembly of cDNA with Illumina paired-end sequencing? I'm really looking for an assembler designed for cDNA data, not DNA. I don't think that programs like Velvet or SSAKE can deal with this kind of data (I may be wrong). MIRA seems OK but it is not quite clear whether it takes the "paired-end" feature into account when assembling cDNA data. Any help is much appreciated !!
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Old 04-01-2010, 09:29 AM   #2
maubp
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I don't understand your question - cDNA is just a small part of a whole genome. Are you talking about an EST assembly of the transcriptome (i.e. extract mRNA, covert to cDNA, sequence it, assemble it).

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Old 04-01-2010, 10:37 AM   #3
Zigster
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yeah I assume he means transcriptome assembly

velvet has an attachment called oases, still in beta, but it seems to produce interesting results. I believe abyss also has something like this in the works.
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Old 04-07-2010, 01:42 AM   #4
mjouret
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Thx Zigster. Yes I mean the transcriptome assembly. After some research, it seems that MIRA can deal with this kind of data (EST + paired end + short read). I saw in several papers that CAP3 was used on the assembled contigs (to get larger contigs). Is this a good idea ? Do you have any advice ?
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Old 04-15-2010, 06:35 AM   #5
Zigster
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if you have 100M short reads I would give those to Velvet and then use those contigs in an alignment based program like you mentioned

i like Mira but the parameter setup is a hassle. Prepare to set aside a couple hours to read the manual.
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assembly, cdna, paired-end, sequencing, solexa

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