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  • BerlindaV
    Junior Member
    • Apr 2014
    • 2

    CDNA synthesis from FFPE: best kit/enzyme?

    This is very much a beginner's question, but does anybody have experience with cDNA synthesis on RNA extracted from FFPE? We would like to ligated our own adaptors to the cDNA, so we need a CDNA synthesis system that does both first and second strand synthesis, and that would work on rather poor quality material.

    Which producers / kits work best in your experience?

    Thank you very much for any advice.

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