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  • Quat
    Junior Member
    • Aug 2011
    • 8

    sequence viewer

    Hello,

    I've been looking around for a while for a visual sequence viewer to replace notepad++ without much luck. The key features I'm interested in are being able to load a fasta file (scaffold/chromosome size hopefully), and being able to either import and view blast alignments, or provide other files for a blast alignment, being able to view coordinates from the match and being able to provide coordinates and extract a sub-sequence from the large fasta file to work with.

    Is there a simple program which fits this bill?

    Thanks
  • bio_boris
    Member
    • Mar 2013
    • 14

    #2
    Have you tried BioEdit? It may have some of the features you are looking for.

    Comment

    • Quat
      Junior Member
      • Aug 2011
      • 8

      #3
      thanks, bioedit is pretty close to what I want and will make things easier.
      hoping to find an editor which will display blast results on a sequence visually.

      if I give it a scaffold as the subject and then blast a coding sequence against the scaffold, I'd like to see the hits laid out on the scaffold.

      Comment

      • bio_boris
        Member
        • Mar 2013
        • 14

        #4
        Doesn't blast give you that information in standard mode?

        Comment

        • Quat
          Junior Member
          • Aug 2011
          • 8

          #5
          I'm not sure what you mean, what I'm familiar with is local blast gives alignments like this:
          Code:
          Query: 403     AQVFHFCI---FGNRLIEESSSVMEAAYSCHWYDGSEEAKTFVQIVCQQCQKAMSISGAK 459
                         + +F F I      +L  +SSSVMEAAYSCHWYDGSEEAKTFVQIVCQQCQK++++SGAK
          Sbjct: 3261942 SMLFFFVICKQLSTQLQFQSSSVMEAAYSCHWYDGSEEAKTFVQIVCQQCQKSLTVSGAK 3261763
          Which are useful, but what I'd like is to be able to see the whole subject strand with the alignments mapped so that I can see possible intron/exon structure easily.

          Comment

          • amitm
            Member
            • Feb 2011
            • 52

            #6
            hi Quat,
            have you tried Jalview. http://www.jalview.org/
            It does very well representation of .aln files

            Comment

            • Quat
              Junior Member
              • Aug 2011
              • 8

              #7
              I hadn't tried jalview, it looks interesting.

              Comment

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