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  • peromhc
    Senior Member
    • Sep 2009
    • 108
    #1

    alternatives to sera-mag bead purification

    Hi All,

    Is anybody out there using something OTHER THAN the Illumina recommended sera-mag beads for mRNA purification?

    The product works fine and all, but it takes so long, and there are many places to mess up.

    I am interested in using a QIAGEN spin column or one of the Invitrogen products for direct mRNA isolation... Anybody using these (or another non-bead system) for Illumina mRNA sequencing?
    Tags: illumina, mrna
    • peromhc
      Senior Member
      • Sep 2009
      • 108
      #2
      Well, I can now say at least that the QIAGEN Oligotrex mRNA purification kit does not work well- at least when following the protocol...

      16% rRNA contamination... this is purifying from total RNA.

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