PubMed: Successful Pyrosequencing of GC-rich DNA Sequences by Partial Substitution of

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PubMed: Successful Pyrosequencing of GC-rich DNA Sequences by Partial Substitution of

05-27-2010, 02:00 AM

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Successful Pyrosequencing of GC-rich DNA Sequences by Partial Substitution of Deoxyguanosine With Deoxyinosine.

Diagn Mol Pathol. 2010 Jun;19(2):123-5

Authors: Bravo LT, Tuohy MJ, Shrestha NK

We experienced significant problems while developing a PyrosequencingTM (Biotage, Uppsala, Sweden) assay to characterize the rifampin resistance-determining region of Mycobacteriumtuberculosis, a target with high guanosine-cytosine content. This paper describes the successful use of a modified pyrosequencing protocol through partial substitution of deoxyguanosine triphosphate with deoxyinosine triphosphate in the polymerase chain reaction.

PMID: 20502190 [PubMed - in process]

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