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  • michaelbarton
    Junior Member
    • Jan 2010
    • 9

    Segmentation fault in consed

    I have assembled sequence data from half a 454 plate. The data was assembled with newbler and appears to have gaps due to problems resolving repeats e.g. repeated rRNA genes. I've heard that consed's autofinish is particularly useful for untangling these areas. I've tried using autofinish on the .ace file generated by newbler, however this returns a segmentation fault error after generating the first set of primers for closing a gap.

    I'm pretty sure I've managed to install consed correctly since I used the biolinux package to do this. Has anyone else encountered this problem?

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
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