Can anyone tell if this HTSeq calculation come out correct

[flavobacteria]

I am a new people in this field. After Bowtie and HTSeq, here is a output .sam file result (just list some here), want to ask your advice:

BD94_4129 0
BD94_4130 0
BD94_4131 0
BD94_4132 0
BD94_4133 0
BD94_4134 0
BD94_4135 0
BD94_4136 0
BD94_4137 0
BD94_4138 0
BD94_4139 0
BD94_4140 0
BD94_4141 0
BD94_4142 0
BD94_4143 0
__no_feature 6117090
__ambiguous 0
__too_low_aQual 0
__not_aligned 1813286
__alignment_not_unique 0



There are some may 0 above the _no_feature line, is it normal, or not suppose to be? Thank you for your advice again!

[dpryan]

You'll always have a good number of 0 counts, for genes not expressed. Having said that, it's good to spot-check a few of these in IGV just to make sure that you did things correctly (at least if you're new to this).