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Old 02-12-2008, 11:30 PM   #1
ECO
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Default Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex

Expanding the ability of next gen sequencers, this group describes a primer bar coding system allowing simultaneous pooling of >1,500 samples.

Check it out here: http://www.ncbi.nlm.nih.gov/pubmed/18264105

Abstract below:

Quote:
Hamady M, Walker JJ, Harris JK, Gold NJ, Knight R.

Department of Computer Science, UCB 430, University of Colorado, Boulder, Colorado 80309, USA.

We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.
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