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Expanding the ability of next gen sequencers, this group describes a primer bar coding system allowing simultaneous pooling of >1,500 samples.
Check it out here: http://www.ncbi.nlm.nih.gov/pubmed/18264105
Abstract below:
Check it out here: http://www.ncbi.nlm.nih.gov/pubmed/18264105
Abstract below:
Hamady M, Walker JJ, Harris JK, Gold NJ, Knight R.
Department of Computer Science, UCB 430, University of Colorado, Boulder, Colorado 80309, USA.
We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.
Department of Computer Science, UCB 430, University of Colorado, Boulder, Colorado 80309, USA.
We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.