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Hi,
In our lab we are working with allele-specific ChIP-seqs and RNA-seqs. After standard ChIP-seq or RNA-seq protocol we attribute obtained reads to either parental or maternal genome based on available SNP information (there is on avarage 1 SNP per 300 bp).
Our department hosts Ion Proton device - and many researchers use it successfully for regular ChIP-seq and RNA-seq experiments.
I would like to get to know if Ion proton is good enough for my purpose.
Does anybody have experience with allele-specific sequencing on Ion Proton?
Is HiSeq2000 better enough to justify paying 2-3 time more for my experiments?
Best,
Michal
In our lab we are working with allele-specific ChIP-seqs and RNA-seqs. After standard ChIP-seq or RNA-seq protocol we attribute obtained reads to either parental or maternal genome based on available SNP information (there is on avarage 1 SNP per 300 bp).
Our department hosts Ion Proton device - and many researchers use it successfully for regular ChIP-seq and RNA-seq experiments.
I would like to get to know if Ion proton is good enough for my purpose.
Does anybody have experience with allele-specific sequencing on Ion Proton?
Is HiSeq2000 better enough to justify paying 2-3 time more for my experiments?
Best,
Michal