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This is the chip i have loaded today.I'm confused why the loading is so bad.As you see in the image,the upper half is so dense,the lower half is so sparse.can anyone tell me the cause?Thanks!
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Newbie here, and I actually use an Ion Proton - but how likely is it that the chip wasn't dry when you loaded the ISPs? It looks like the sample entered the chip by displacing another solution - in this case the two blue "vortexes" and the bottom part of the chip would be where the solution lingered.
Alternatively... a warped chip, perhaps?Last edited by r.rosati; 08-05-2015, 03:44 PM. -
Originally posted by r.rosati View Post
Thanks for your answer!I'll try your advice next time!Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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