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Like many folks working on low diversity libraries, we have been hit hard by the problems associated with the V3 kit - lots of time and effort wasted. Is this problem also common for high diversity libraries? I would appreciate any insights.
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I've had problems with high-diversity (gDNA) libraries with the 600-cycle V3 kits, even with a cluster density of 1K. Read 2 just tanked after about 50 bp. We are advising people not to run them at all until Illumina sorts out whatever problem this is. -
Thank you! Will it be possible to mix our MiSeq libraries with our HiSeq libraries (e.g., RNA-Seq, have not started yet) and running the mix libraries with HiSeq2500 or 3000?Comment
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I've run libraries on the MiSeq (usually for troubleshooting purposes) and then loaded them on a HiSeq once I was sure everything was okay with them. The loading concentrations are usually different, in my experience, but that should be the only issue you'll need to address.Comment
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Good to know-thanks!Comment
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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