Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • huan
    Member
    • Oct 2010
    • 56

    #1

    How can I calcualte the sequence identity and coverage form GMAP output bam file?

    Now I have got a bam file from gmap alignment with data from pacbio isoseq result. Now I want to get the statistic information about the coverage and identity of every sequence. So is there any tools can do the stat?Or How can I compute the identity and coverage form sam file?
    Thanks a lot for reply!
    Last edited by huan; 01-20-2016, 12:37 AM.
    happy
  • Brian Bushnell
    Super Moderator
    • Jan 2014
    • 2709

    #2
    The BBMap package calculates both coverage and identity. For coverage, you can calculate it using a bam file with pileup.sh. Identity, however, requires alignment via BBMap (bbmap.sh) - just add the "idtag" flag, and all alignments will have an additional field indicating their percent identity, prefixed by "YI:f:". bbmap.sh can generate coverage and identity at the same time.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
      by SEQadmin2



      CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

      Despite this, “CRISPR helped turn genome editing from a specialized technique into
      ...
      07-31-2026, 11:01 AM
    • SEQadmin2
      Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
      by SEQadmin2


      Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

      The systematic characterization of the human proteome has
      ...
      07-20-2026, 11:48 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, Yesterday, 12:22 PM
    0 responses
    15 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 08-11-2026, 10:35 AM
    0 responses
    16 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 08-06-2026, 07:41 AM
    0 responses
    31 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 08-03-2026, 10:13 AM
    0 responses
    50 views
    0 reactions
    Last Post SEQadmin2  
    Working...