Disproportionate Ratio of Index Adapters

[hideandSEQ]

So my protocol is using a custom P5 index adapter with a standard Illumina Nextera N70X oligo. I made the mistake of using a P5 aliquot that is somewhere between 10 and 100x more concentrated than it should have been.

Aside from primer dimer formation which I can fix, are there any other potential issues this may have caused?

I need to decide whether I should toss these libraries and run tagmentation on a new batch, or send this batch to sequencing after PCR purification.

[Jessica_L]

I think you might be okay? If possible, a bioanalyzer run on the completed libraries could help you figure out if there are improperly formed products and if the size of the main insert/library is correct. I'd say that if everything looks as expected, you should be okay to proceed to sequencing?