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  • rvgill
    Junior Member
    • Oct 2016
    • 6

    getting .nal files from blast

    Hello,

    So I am making my own databases from fasta files using Blast version ncbi-blast-2.5.0+.dmg and getting an error that is linking back to by database creation by not having a .nal file created.
    I am entering in:
    makeblastdb -in test2.fasta -out MV1SDB_a/MV1SDB_a -parse_seqids -dbtype nucl

    And getting the following files outputs:
    MV1SDB_a.nhr
    MV1SDB_a.nin
    MV1SDB_a.nog
    MV1SDB_a.nsd
    MV1SDB_a.nsi
    MV1SDB_a.nsq

    How do I change my coding to recieve a .nal file as one of my outputs.
  • maubp
    Peter (Biopython etc)
    • Jul 2009
    • 1544

    #2
    This is a near duplicate of your other thread where you have been given advice and help: http://seqanswers.com/forums/showthread.php?t=72083

    The .nal file is a plain text file describing how to treat a set of BLAST nucleotide database "chunks" as a single database. When making a database from a single FASTA file this won't happen (unless your FASTA file has an enormous number of sequences).
    Last edited by maubp; 10-26-2016, 07:11 AM. Reason: fixed markup

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