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Old 06-15-2017, 01:59 PM   #1
Location: cambridge,UK

Join Date: Dec 2014
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Default is this a technical replicate or biological replicate

i know that biological replicate is one taken from different organism/sample while technical replicate is one from same organism/sample.

However, I am not able to understand that a cell line treated with a drug three times separately to generate RNA-Seq data will be considered as biological replicate or technical replicate or a biological replicate but not in a strict sense. Also, will this be applicable for control sample?

Thanks in advance
imsharmanitin is offline   Reply With Quote
Old 06-15-2017, 02:58 PM   #2
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I think it would be biological replicates and the same goes for control which should be treated with solvent without the drug. I imagine that there will be other treatments such as exposure time and dose as well in such experiments.
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Old 11-28-2017, 07:19 AM   #3
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As the variation during cultering is expected (and often is) far more variable then the technical reproducibility the biological replicate would be to repeat the actual incubations i.e. three times (for a triplicate).
A technical replicate would be having the same RNA sample sequenced two times (i.e. a duplo).
Often an experiment is repeated three times, getting n=3 for the experiment and statistics. In the experiment an incubation can be in duplo (i.e. two flasks stimulated with the same compound). Each incubation is then sequenced once.
jaspersaris is offline   Reply With Quote

cell line, replicates, rna-seq, rna-seq advice, rna-seq design

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