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Does anyone have experience with SeqCap EZ HyperCap Workflow for Target Enrichment protocol from Roche? I'm unable to find any public datasets I can look into. Any info or links to the data is highly appreciated. Thanks in advance!
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This is the main page for the SeqCap EZ HyperCap site:
Was there anything in specific you are seeking? The User's Guide link is embedded halfway down the page, prior to the data presented.
Full disclosure: Roche is my employer.Full Disclosure: Roche employee -
I'm looking for the user data or experiences. I can find plenty of data (at SRA) for SeqCap EZ, but none for SeqCap EZ HyperCapComment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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