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Old 11-09-2017, 01:18 AM   #1
Junior Member
Location: Trondheim, Norway

Join Date: Mar 2017
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Default QIAseq targeted DNA panel for cfDNA analysis

I would like to get in touch with someone who have used QIAseq targeted DNA panel. We are struggling with cfDNA libraries and I am wondering if anyone are experiencing the same?
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Old 01-16-2018, 11:07 AM   #2
Location: Santa Cruz, CA

Join Date: Feb 2017
Posts: 17

I do not have experience with QIAseq targeted DNA panel. But can I ask what struggles do you have with cfDNA libraries?

How big is the panel that you are trying to profile?

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Old 05-13-2019, 01:03 AM   #3
ecSeq Bioinformatics
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Location: Leipzig, Germany

Join Date: May 2012
Posts: 276

Hi everyone,

our genetic testing software 'Seamless NGS' offers an easy-to-use workflow for the analysis of Qiagen's QiaSeq panels.

Before mapping the reads, the common sequence and the UMIs are clipped away. After mapping, the UMIs sequences are properly used to correct for PCR clones and the single amplicon primer is clipped away to ensure correct and unbiased variant calling. The workflow was developed, tested and validated together with a trustworthy pathological laboratory at one of the oldest university hospitals in Germany. Having long-lasting experience in that field, together with them, ecSeq was able to get the most out of the QiaSeq panel.

If you are interested in our solution, please check and/or write us a short email ( to make an appointment for a personal live demo (online).

ecSeq Bioinformatics is Europe’s leading provider of hands-on bioinformatics workshops and professional data analysis in the field of Next-Generation Sequencing (NGS).

Last edited by ecSeq Bioinformatics; 05-13-2019 at 01:15 AM.
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