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  • mapper
    Member
    • Nov 2010
    • 17

    Uniquely mapped reads with bowtie

    Hi all

    Is there anyway to report only mapped tags in bowtie sam output?

    I tried to map reads with bowtie with -m 1 --best and --strata parameter but in mapping output(sam) reads which are not aligned are also reported...
  • frozenlyse
    Senior Member
    • Sep 2008
    • 135

    #2
    I filter them out using samtools whilst converting to BAM on the fly eg

    bowtie -m 1 --your-other-settings - | samtools view -F 4 -S -b - > output.bam;

    Comment

    • mapper
      Member
      • Nov 2010
      • 17

      #3
      thnx ........

      Comment

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