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  • PFS
    Member
    • Mar 2010
    • 55

    transcript length bias in enrichment analysis and RPKM

    I read about the need of correcting for transcript length bias when performing GO enrichment analysis in RNA-Seq data.

    If we use RPKM values instead of raw counts, is this still true?
  • frozenlyse
    Senior Member
    • Sep 2008
    • 135

    #2
    No - check out this reference which goes through the reasoning and tries to address the problem though http://genomebiology.com/content/11/2/R14

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