Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • tickteng
    Junior Member
    • Apr 2019
    • 6

    Newbie: Primer with index

    Hi everyone, kinda new to the whole sequencing. So I want to run multiple sample in minion. There is barcode provided by oxford nanopore, but i also would like to attach index to my pcr.

    I am planning to make 18 or 22 bp of index.
    The question is, is there any platform that you use to design index? or is it just randomly decided by you?
  • Marc_Jones
    Member
    • Jun 2017
    • 10

    #2
    You could just add the index to the primers when you order them.

    or

    SQK-RPB004 or SQK-PBK004 depending on your experiment.

    Comment

    • tickteng
      Junior Member
      • Apr 2019
      • 6

      #3
      hmm, not really like that. So beside the one provided by nanopore, I plan to attach some kind of index to the primer itself, so the result can be divided further in one barcode.

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by SEQadmin2


        I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.


        Here are nine questions we think about, in roughly the order they matter, before...
        06-18-2026, 07:11 AM
      • SEQadmin2
        From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
        by SEQadmin2


        Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


        The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
        ...
        06-02-2026, 10:05 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 06-17-2026, 06:09 AM
      0 responses
      30 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-09-2026, 11:58 AM
      0 responses
      96 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-05-2026, 10:09 AM
      0 responses
      115 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 06-04-2026, 08:59 AM
      0 responses
      109 views
      0 reactions
      Last Post SEQadmin2  
      Working...