Tweet
Is it possible to increase the number of samples of NextSeq 500 v2.5 high output flow cell beyond the recommended amount of 12 samples for exome sequencing? Has it been done before?
-
-
As long as you have a unique set of index sequences it is certainly possible. The total yield from a FC is not going to change so that final read number will get divided into the samples you put in the pool i.e. each sample will get proportionately less number of reads. -
Thank you for your reply GenoMaxComment
-
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
Comment