Alternative to Qiagen Puregene Tissue kit for HMW gDNA?

[dacotahm]

Hello,

We're extracting high molecular weight gDNA for PacBio from an insect-a bee- using the Qiagen Puregene Tissue kit (4g). We have trouble with this kit back-ordered all the time. Could someone from the community recommend an alternative for HMW gDNA that works well on tissue for PacBio?

Thanks,

Dacotah

[ATϟGC]

I have tried the following protocol with insects and it works quite well in terms of purity and length:

Modified low‐salt CTAB extraction of high‐quality DNA from contaminant‐rich tissues

https://onlinelibrary.wiley.com/doi/full/10.1111/1755-0998.12616

A benchtop protocol is available in the "Supporting Information" section.

[olafblue1955]

Hi Dacotah,

Qiagen's PureGene kits are nearly identical to the former Epicentre MasterPue DNA Purification kits, which are now available through Lucigen/Biosearch Technologies. Catalog numbers would be MC85200 for 200 reactions and MC89010 for 10 reactions. Both are non-column based products.

Olafblue1955

[dacotahm]

Oh, that's great, thank you very much.