Nanoarray sequenzing, cluster-plot

Mare148

Junior Member

Join Date: Sep 2020

Posts: 1
- Share
- Tweet
#1

Nanoarray sequenzing, cluster-plot

09-12-2020, 09:26 AM

IMG-6458 hosted at ImgBB

https://ibb.co/9qbR0st

Image IMG-6458 hosted on ImgBB

I don't understand picture B, it`s an Cluster plot of normalized intensities from a high-density test array with 700nm center-to-center spot distance. This array has 3,4 fold more DNA spots per image than 1,29 micorn arrays. It uses only 4,2 pixels per spot and generates similar raw base discordances.

Thanks for your help
Tags: bioinformatics, clustering, nanoarrays, sequenzing
snetmcom

Senior Member

Join Date: Oct 2008

Posts: 159
- Share
- Tweet
#2

09-15-2020, 08:22 PM

B looks like an old satay plot from SOLiD sequencing days. They were used to measure signal strength, purity, and phasing. Dots on each axis line are pure colors with no mixed clusters. Dots furthest from the origin on each axis are higher signal or intensity.
Comment

Previous template Next

Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems

by SEQadmin2

Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
...
- Channel: Articles
07-09-2026, 11:10 AM
Cancer Drug Resistance: The Lingering Barrier to Rising Survival

by SEQadmin2

Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.

There is no single reason why many patients don’t respond to treatment as expected. Cancer is...
- Channel: Articles
07-08-2026, 05:17 AM
Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing

by GATTACAT

Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
- Channel: Articles
07-01-2026, 11:43 AM

Topics	Statistics	Last Post
UC San Diego Bioengineers Map Gene Function in Human Stem Cells by SEQadmin2 Started by SEQadmin2, 07-13-2026, 10:26 AM	0 responses 28 views 0 reactions	Last Post by SEQadmin2 07-13-2026, 10:26 AM
New Analysis Splits Leukemia Into 16 Epigenomic Subgroups by SEQadmin2 Started by SEQadmin2, 07-09-2026, 10:04 AM	0 responses 37 views 0 reactions	Last Post by SEQadmin2 07-09-2026, 10:04 AM
Genome-Wide CRISPR Screen Uncovers Unlikely Psoriasis Target by SEQadmin2 Started by SEQadmin2, 07-08-2026, 10:08 AM	0 responses 25 views 0 reactions	Last Post by SEQadmin2 07-08-2026, 10:08 AM
Engineered Protein Motor Takes Its First Steps Along DNA Track by SEQadmin2 Started by SEQadmin2, 07-07-2026, 11:05 AM	0 responses 35 views 0 reactions	Last Post by SEQadmin2 07-07-2026, 11:05 AM

Unconfigured Ad

Nanoarray sequenzing, cluster-plot

Comment

Latest Articles

ad_right_rmr

News