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Dear everybody,
I'm trring to understand how solexa sequencing works. Probably what I'm doing it's really a very stupid question, but I'm not able to understand it.
The basic protocol for chip-seq should be:
making chromathinIP;
cut the DNA with enzymes making 30bp fragments;
then ligate it;
amplify;
fix to a support;
sequence it;
map the tags and etc.
It's assumed that the tags (30bp fragments) are unique or the + sense or at the - sense, but I don't understand how it is possible. Are thery really unique or we choose them as unique???? Thanks a lot for your answer and sorry for the stupid question,
Best
Fabio
I'm trring to understand how solexa sequencing works. Probably what I'm doing it's really a very stupid question, but I'm not able to understand it.
The basic protocol for chip-seq should be:
making chromathinIP;
cut the DNA with enzymes making 30bp fragments;
then ligate it;
amplify;
fix to a support;
sequence it;
map the tags and etc.
It's assumed that the tags (30bp fragments) are unique or the + sense or at the - sense, but I don't understand how it is possible. Are thery really unique or we choose them as unique???? Thanks a lot for your answer and sorry for the stupid question,
Best
Fabio