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16s alignment annotation assembly atac-seq bam bbmap bedtools bioinfomatics bioinformatics bioinformatics analysis blast bowtie bowtie2 bwa cancer chip-seq chipseq cnv coverage cuffdiff cufflinks cummerbund de novo assembly deseq deseq2 differential expression edger epigenetics error fastq genome genomics hisat2 hiseq htseq count illumina job jobs mapping metagenomics methylation microbiome mirna miseq nanopore ngs pacbio pipeline python rna rna-seq rna-seq analysis rna-seq data analysis rnaseq rnaseq analysis rna sequencing sam samtools sequencing snp software star tophat tophat2 vcf vcftools webinar wgs workshop

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