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16s alignment annotation assembly atac-seq bam bbduk bbmap binning bioinfomatics bioinformatics bioinformatics analysis blast bowtie2 bwa cancer cnv coverage cuffdiff cufflinks cummerbund de novo assembly deseq deseq2 differential expression edger epigenetics error fastq genome genomics hisat2 hiseq illumina job jobs mapping metagenomics methylation microbiome mirna miseq nanopore ngs pacbio pipeline python rna rna-seq rna-seq advice rna-seq analysis rnaseq rnaseq analysis rna sequencing sam samtools sequencing snp software spike-in control star tcga tophat trimmomatic variant calling vcf vcftools webinar wgs workshop

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