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16s alignment annotation atac-seq bbmap bcftools bioanalyzer bioinformatics bioinformatics software blast blastp bwa command line competition crispr data science demultiplexing deseq2 differential expression divergent reference dna dna-seq dna extraction fasta fastq.gz fastq files genome annotation genome assembly genome size estimation genomics gtf annotation file igv illumina introduction kegg library generation library prep library qc liquid biopsy machine learning mapping metagenomics midwest miseq miseq v3 multiplexing nanopore new to next gen nextseq ngs novaseq pacbio primer purdue rna-seq rnaseq samtools scrnaseq sequencing seurat single cell rna seq snrnaseq spades stampy error tapestation vcf webinar wgs workflows workshop

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