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16s alignment annotation assembly bam bbduk bedtools bioanalyzer bioinfomatics bioinformatics blast bowtie 2 bowtie2 bwa cancer chip-seq chipseq chip seq cnv cuffdiff cufflinks cummerbund database de novo assembly deseq deseq2 differential expression edger epigenetics error fasta fastq genome genomics hisat2 hiseq htseq count illumina job jobs mapping metagenomics microbiome mirna miseq ngs normalization pacbio pipeline python rna rna-seq rna-seq advice rna-seq analysis rnaseq rnaseq analysis rna sequencing rsem sam samtools sequencing software spike-in control star tophat tophat2 vcf velvet wgs workshop

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