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16s alignment assembly atac-seq bam bbmap bioinfomatics bioinformactics bioinformatics bowtie bwa cancer chip-seq coverage cuffdiff cufflinks de novo assembly deseq2 differential expression dna-seq dnaseq edger epigenetics error fastq fastqc genome genome assembly genomics hisat2 illumina illumina miseq index ion torrent job library mapping merge metagenomics microbiome minion mirna miseq ngs ngs data analysis pacbio python rna rna-seq rna-seq advice rna-seq analysis rna-seq data analysis rnaseq rnaseq analysis rna sequencing sam samtools sequencing snp software splicing star tcga tophat trimming trimmomatic variant calling vcf webinar workshop

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