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News about the latest advances and discoveries in sequencing and related technologies.
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Dealing with customer samples, data, and the challenges that come with both?
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Literature Watch
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Complete Genomics
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
Topics: 57
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Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
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Weird 16S custom primer issue on Aviti runs
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Illumina (Solexa)
Bridged amplification & clustering followed by sequencing by synthesis.
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Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
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Oxford Nanopore
Single-molecule sequencing via a protein nanopore.
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Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
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Singular Genomics
Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
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Ultima Genomics
Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
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Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
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Service Providers
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Archived Forums
A place for discussion of older forums and sequencing technologies.
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Bioinformatics
Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
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Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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Single Cell Sequencing
Techniques, discussions, and applications of single cell sequencing.
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Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
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De novo discovery
Wandering without a reference? Post here
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Metagenomics
Ever wonder what's growing in that hot spring or glacier?
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Epigenetics
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
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RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
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Clinical Sequencing
Discuss issues unique to clinical sequencing.
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Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
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Childhood cancers, effectively fatal 60 years ago, are now curable for up to 85% of children with access to contemporary treatments and...05-08-2026, 01:42 AM -
In this SEQanswers webinar, three leaders in genomics and computational biology—Mark Robinson, Adam Cribbs, and Athma A. Pai—will discuss...02-13-2026, 06:49 AM -
In this joint webinar, experts from Illumina, Twist Bioscience, and Enpicom will explore an end-to-end workflow for accelerating scFv discovery,...02-05-2026, 11:53 AM
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Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...Today, 05:17 AM -
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before... -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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