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Introductions
New here? Stop in and introduce yourself. Where you are, what you work on, etc.
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Last Post: Hi ! I'm Nouara
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Sunflower34
Sequencing Resources
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News
News about the latest advances and discoveries in sequencing and related technologies.
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Dealing with customer samples, data, and the challenges that come with both?
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128 581
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1,927 7,109
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Literature Watch
Discussion of any scientific study related to high content or next generation genomics. Whole genome association, metagenomics, digital gene expression, etc.
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1,536 2,216
Complete Genomics
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
Topics: 52 Posts: 164
Last Post: 69 Genomes Data
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Sarp
by Sarp
Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
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2 5
Illumina (Solexa)
Bridged amplification & clustering followed by sequencing by synthesis.
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3,067 16,768
Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
Topics: 388 Posts: 2,175
Last Post: Proton Data
388 2,175
ehaag
by ehaag
Oxford Nanopore
Single-molecule sequencing via a protein nanopore.
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126 611
Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
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389 2,182
Singular Genomics
Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
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0 0
Ultima Genomics
Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
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Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
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78 288
Miranda
by Miranda
Service Providers
Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.
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75 477
Archived Forums
A place for discussion of older forums and sequencing technologies.
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Last Post: Rapid Libraries
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pmiguel
by pmiguel
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Bioinformatics
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Bioinformatics
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22,069 103,101
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vivianshaw
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Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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Single Cell Sequencing
Techniques, discussions, and applications of single cell sequencing.
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8 15
Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
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280 994
De novo discovery
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340 1,699
Metagenomics
Ever wonder what's growing in that hot spring or glacier?
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268 847
Epigenetics
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
Topics: 357 Posts: 1,578
357 1,578
RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
Topics: 2,865 Posts: 11,429
2,865 11,429
Clinical Sequencing
Discuss issues unique to clinical sequencing.
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38 104
Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
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60 294
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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.


    Here are nine questions we think about, in roughly the order they matter, before...
    Today, 07:11 AM
  • SEQadmin2
    From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data


    Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


    The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
    ...
    06-02-2026, 10:05 AM
  • SEQadmin2
    Single-Cell Sequencing at an Inflection Point: Early Impacts of New Platforms and Emerging Trends


    With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.


    Introduction

    Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...
    05-22-2026, 06:42 AM

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