Hi,
we have some plant RNA samples obtained from a phenol-chloroform extraction followed by LiCl precipitation; we would like to sequence these RNA samples with Illumina Hiseq platform, however RIN ratios are all between 5.5 and 6.5 (I’ve attached a typical bioanalyzer result, Nanodrop values for this sample are 2.14 for 260/280 ratio and 2.41 for 260/230 ratio)
Does anyone know what kind of contamination we have in our samples and to what kind of problems this could lead if we tried to sequence this sample? purification with phenol-choloform and ethanol precipitation does not lead to better results, any idea if there's another possibility to purify the samples?
Thanks a lot for comments - suggestions!
we have some plant RNA samples obtained from a phenol-chloroform extraction followed by LiCl precipitation; we would like to sequence these RNA samples with Illumina Hiseq platform, however RIN ratios are all between 5.5 and 6.5 (I’ve attached a typical bioanalyzer result, Nanodrop values for this sample are 2.14 for 260/280 ratio and 2.41 for 260/230 ratio)
Does anyone know what kind of contamination we have in our samples and to what kind of problems this could lead if we tried to sequence this sample? purification with phenol-choloform and ethanol precipitation does not lead to better results, any idea if there's another possibility to purify the samples?
Thanks a lot for comments - suggestions!
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