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Hi Every one
I m new in RNA seq study. I m using Tophat and cufflink pipeline for referenced based RNA seq study and differential gene expression. now i want to extract or get alternative splice transcript how can i get alternative asplice transcript from my output files. i performed Tophat-cufflink-cuffcompare and cuffdiff. but i dont know how can i will get my alternative splice transcript from my output file?? Can anybody suggest me how can i get?? Any suggestions are appreciated. Thank in advance.
I m new in RNA seq study. I m using Tophat and cufflink pipeline for referenced based RNA seq study and differential gene expression. now i want to extract or get alternative splice transcript how can i get alternative asplice transcript from my output files. i performed Tophat-cufflink-cuffcompare and cuffdiff. but i dont know how can i will get my alternative splice transcript from my output file?? Can anybody suggest me how can i get?? Any suggestions are appreciated. Thank in advance.