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  • How to get gene symbol after deseq?

    Dear experts,

    After I have run deseq, I got a list of genes. They are something like as follow.

    > resSig[,1]
    [1] "ENSMUSG00000022150+ENSMUSG00000079102:009"
    [2] "ENSMUSG00000026727:016"
    [3] "ENSMUSG00000026727:004"
    [4] "ENSMUSG00000022150+ENSMUSG00000079102:015"
    [5] "ENSMUSG00000025730:010"
    [6] "ENSMUSG00000005836:007"
    [7] "ENSMUSG00000073139:001"

    How can I get back the gene symbol for each ID?

    Thank you very much in advance.

  • #2
    Hi,
    When counting mapped reads using HTseq, you need to use gene symbol insteads of gene_id (this is default)

    Comment


    • #3
      Use merge with your annotation and edgeR or DESeq result files.

      Originally posted by fabrice View Post
      Dear experts,

      After I have run deseq, I got a list of genes. They are something like as follow.

      > resSig[,1]
      [1] "ENSMUSG00000022150+ENSMUSG00000079102:009"
      [2] "ENSMUSG00000026727:016"
      [3] "ENSMUSG00000026727:004"
      [4] "ENSMUSG00000022150+ENSMUSG00000079102:015"
      [5] "ENSMUSG00000025730:010"
      [6] "ENSMUSG00000005836:007"
      [7] "ENSMUSG00000073139:001"

      How can I get back the gene symbol for each ID?

      Thank you very much in advance.
      If you have annotation file you can try merging the columns using column names.

      I have also attached sample input and sample output files.


      dbs <- read.table("sample_annotation.txt", header=TRUE)
      resR <- read.table(edgeR_results.txt,header=TRUE)
      newy <- cbind(rownames(resR),resR)
      colnames(newy)[1] <- "ID"
      annotated <- merge( dbs, newy, by='ID')
      write.table(annotated,edgeR_results_annotated.txt,sep=" \t")


      I hope this will help you.
      Attached Files

      Comment

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