Hi
I have got the adapter sequences from company as for Illumina RNA seq data:
5-AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGACGATC-(insert)-ACCTTAAGAGCCCACGGTTCCTTGAGGTCAGTGXXXXXXTAGAGCATACGGCAGAAGACGAAC-3
(ID #1 "ATCACG" )
Will this be my adapter sequences to trim it using trimmomatic?
>adpter1
AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGACGATC
>adapter2
ACCTTAAGAGCCCACGGTTCCTTGAGGTCAGTGATCACGTAGAGCATACGGCAGAAGACGAAC
or I have to reverse complement the adapter ?
Best Regards
I have got the adapter sequences from company as for Illumina RNA seq data:
5-AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGACGATC-(insert)-ACCTTAAGAGCCCACGGTTCCTTGAGGTCAGTGXXXXXXTAGAGCATACGGCAGAAGACGAAC-3
(ID #1 "ATCACG" )
Will this be my adapter sequences to trim it using trimmomatic?
>adpter1
AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGACGATC
>adapter2
ACCTTAAGAGCCCACGGTTCCTTGAGGTCAGTGATCACGTAGAGCATACGGCAGAAGACGAAC
or I have to reverse complement the adapter ?
Best Regards
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