UTR region misassembly?

[wyim]

Dear folks,

Helloo!

During my RNA-seq assembly, I found many misassemble or SNP or indel in UTR region.

I used SOAPdenovo-trans, Trinity and others.

They always generated lots of redundancy.

And this redundancy generated from UTR regions even though the gene body have well consensus sequence.

Cause of this UTR difference, I think they generated a lot of redundancy.

Do you have any idea of this happening?

I think the coverage of UTR is lower than gene body.

Please give me some reference.



Thank you.