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  • nareshmvr
    Member
    • Apr 2011
    • 16

    RNA-Seq vs qRT-PCR

    I am attaching a figure based on assessing qRT-PCR vs Dual-RNASeq for selected Genome1 genes (black circles) and Genome2 genes (red circles).

    I have analysed the correlation by Spearman Rank Order Correlation analysis:

    Correlation Coefficient: 0.727
    P Value: 0.00946
    Number of Samples: 11

    So, overall there is a significant correlation between the two datasets.

    BUT, there is something slightly strange going on here. For Genome1, RNASeq seems to underestimate the changes in gene expression compared with qRT-PCR. Genome-2, it is the opposite - RNASeq overestimates gene regulation.
    I have absolutely no idea why RNASeq should specifically underestimate Genome-1 gene regulation. please give your valuable suggestions in interprating for this qRT-PCR Validations
    Attached Files
    Last edited by nareshmvr; 05-17-2016, 07:56 PM.
  • nareshmvr
    Member
    • Apr 2011
    • 16

    #2
    Dear All,

    Am still wondering about this issue , I think my question is not unclear , Please suggest me if there are any possible ways to debug my issue.

    Comment

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