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We are trying to troubleshoot contamination issues that we are having in our ChIP-seq experiments. It seems that recently we have been sequencing constructs that have been cloned in the lab in other experiments in our IPd samples. We are trying to decided how to proceed, how many precautions to take. We are interested in hearing what others do. Do you have a designated area, designated equipment, etc.? At what point in the procedure do you begin to use this designated area? As early as the IP or just at the amplification step? Any and all of your thoughts and suggestions would be greatly appreciated!
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Many organizations study rare diseases, but few have a mission as impactful as Rady Children’s Institute for Genomic Medicine (RCIGM). “We are all about changing outcomes for children,” explained Dr. Stephen Kingsmore, President and CEO of the group. The institute’s initial goal was to provide rapid diagnoses for critically ill children and shorten their diagnostic odyssey, a term used to describe the long and arduous process it takes patients to obtain an accurate...12-16-2024, 07:57 AM -
Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.
Long-Read Sequencing
Long-read sequencing has seen remarkable advancements,...12-02-2024, 01:49 PM