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  • How does new Truseq Stranded Protocol actually work? Is it dUTP based??

    How does new Truseq Stranded Protocol actually work? Is it dUTP based??
    I have not done this work. Please advise. thank you very much.

  • #2
    Yes it is dUTP based. Also adds actinomycin D to first strand synthesis, presumably to prevent any second strand synthesis during that step.

    --
    Phillip

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    • #3
      Philip, Thank you very much clarifying this. Did you use this in your laboratory? and how happy are you with this?
      thank you.

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      • #4
        Have a kit, did not try it yet. Next week, maybe.

        --
        Phillip

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