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We want to use the ScriptSeq v2 RNA-seq Library prep kit (Epicentre) to make libraries for some rRNA-depleted bacterial RNA. Looking through the protocol I don't see any option for a size-selection. We'd like to size-select because we have a lot of small RNA product that we don't want to include in the prep. Has anyone incorporated a size-selection into the protocol? I would think you could add it after the completed cDNA synthesis and tagging (after 3.C in current protocol). Would you still do the cDNA purification (3.D in current protocol) if there is a size-selection after cDNA synthesis? We have access to a pippin prep if that will be useful.
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The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...11-06-2024, 07:24 PM -
Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...10-18-2024, 07:11 AM