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  • Major bottlenecks in NGS sample prep

    Hi all!

    I'm curious, what are the major bottlenecks for you in NGS library preparation? Does automation solve them?

  • #2
    Sample QC before library prep is the major bottleneck for us

    Comment


    • #3
      sara_ahmed, could you please explain more, what issue do you have with the sample QC? Do you work with DNA or RNA?

      Comment


      • #4
        We work with both. It is not necessarily a problematic step, just the slow step when dealing with a high volume of samples. It is also not solved easily by automation.

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        • #5
          Fragmentation of genomic DNA if you have a single sample covaris!
          Any kind of gel extraction.

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